Coding

Part:BBa_K2957013

Designed by: Ethan Levy   Group: iGEM19_MIT   (2019-09-22)


CCL5 with NeonGreen tag (C-terminal)

Contents: CCL5 (RANTES), NeonGreen tag, with Carb resistance

What is it?: A Basic part; in this particular case we call it a pL0-G (through Modular Cloning Golden Gate Type II Assembly)

What does it do?: It codes for the chemokine, CCL5, which is a chemoattractant for T cells. It was important for our project in particular because of its involvement in the immune system. It has a NeonGreen tag attached.

How to use it?: You can use this part for Type IIS Cloning. It has BsaI cut sites that allows for this. With other Basic parts like pL0-P (promoter/regulatory sites), pL0-T (terminator part), pL0-5.2/pL0-3 (Inert pieces that form the UTR regions around the genetic circuit), you can use this part to make a composite part. So the pL0's are placed in a given backbone depending on the kind of basic part it is. In this case, we have the CDS/gene part which is in a pL0-G backbone. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>."

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 442
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 442
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 458
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 442
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 442
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None